Journal: Advances in Radiation Oncology

Article Title: Kupffer Cell-Derived Interleukin-6 Aggravates Radiation-Induced Liver Disease by Activating Hepatocyte STAT3 to Promote Ccng1 Transcription

doi: 10.1016/j.adro.2026.102003

Figure Lengend Snippet: Blockade of classical IL-6 signaling alleviates radiation-induced liver disease (RILD) in rats. (A) Quantitative enzyme-linked immunosorbent assay (ELISA) analysis of interleukin (IL)-6 protein concentrations in peripheral serum and liver homogenates from Ctrl and irradiation (IR) rats (n = 5). (B) Quantitative ELISA analysis of serum liver enzymes in Ctrl rats or IR rats treated with placebo, anti-IL-6, or sgp130Fc (n = 5). (C) Hematoxylin–eosin (H&E) staining of rat livers. (D) F4/80 staining (red) showing Kupffer cell infiltration in rat livers. (E) Myeloperoxidase (MPO) staining (red) revealed neutrophil infiltration in the rat liver. (F) Costaining of CD31 (red), HNF4α (blue), and TUNEL (green) in rat livers. Student’s t test or analysis of variance (ANOVA); ⁎⁎⁎ , P < .001; ns, nonsignificant.

Article Snippet: TUNEL staining was performed using a TUNEL BrightGreen Apoptosis Detection Kit (Vazyme, #A112-03) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation, Staining, TUNEL Assay

Journal: Advances in Radiation Oncology

Article Title: Kupffer Cell-Derived Interleukin-6 Aggravates Radiation-Induced Liver Disease by Activating Hepatocyte STAT3 to Promote Ccng1 Transcription

doi: 10.1016/j.adro.2026.102003

Figure Lengend Snippet: Role of the JAK–STAT signaling pathway in hepatocytes during radiation-induced liver disease (RILD). (A) Volcano plot of differential gene expression analysis in hepatocytes after irradiation (IR). Red and blue indicate upregulated and downregulated genes, respectively. The dotted horizontal line represents a P value of 0.05. The dotted vertical lines represent a log2-fold change of 1.5 or −1.5. (Right) Bubble chart depicting the top 5 Kyoto Encyclopedia of Genes and Genomes (KEGG)-enriched pathways corresponding to down/upregulated genes in hepatocytes after IR (screening the pathways and sorting them from large to small according to the −log 10 P value). (B) Western blot analysis showing p-STAT3 and GAPDH expression in primary hepatocytes isolated from Ctrl rats or IR rats treated with placebo, anti-interleukin (IL)-6, or sgp130Fc (n = 5). (C) Western blot analysis showing p-STAT3 and GAPDH expression in primary hepatocytes isolated from IR rats treated with placebo, ruxolitinib (RUX), or tofacitinib (TOF) (n = 5). (D) Quantitative enzyme-linked immunosorbent assay (ELISA) analysis of serum liver enzymes in IR rats treated with placebo, RUX, or TOF (n = 5). (E) Hematoxylin–eosin (H&E) staining of the livers of IR rats treated with placebo, RUX, or TOF (n = 5). (F) Costaining of HNF4α (red) and TUNEL (green) in the livers of IR rats treated with placebo, RUX, or TOF (n = 5). (G) Overlap of p-STAT3-binding genes identified using ChIP-Seq with upregulated genes ( P < .05 and log2-fold change > 2) in hepatocytes identified using scRNA-seq. (H) Violin plots showing the expression levels of overlapping genes identified using scRNA-seq. (I) qRT‒PCR analysis of overlapping genes in isolated primary hepatocytes from IR rats treated with placebo, RUX, or TOF (n = 5). Analysis of variance (ANOVA); *, P < .05; ⁎⁎ , P < .01; ⁎⁎⁎ , P < .001; ns, nonsignificant.

Article Snippet: TUNEL staining was performed using a TUNEL BrightGreen Apoptosis Detection Kit (Vazyme, #A112-03) according to the manufacturer's instructions.

Techniques: Gene Expression, Irradiation, Western Blot, Expressing, Isolation, Enzyme-linked Immunosorbent Assay, Staining, TUNEL Assay, Binding Assay, ChIP-sequencing

Journal: Advances in Radiation Oncology

Article Title: Kupffer Cell-Derived Interleukin-6 Aggravates Radiation-Induced Liver Disease by Activating Hepatocyte STAT3 to Promote Ccng1 Transcription

doi: 10.1016/j.adro.2026.102003

Figure Lengend Snippet: Schematic of the Kuppfer cell (KC)-hepatocyte crosstalk mechanism in RILD. Irradiation (IR) stimulates KCs to secrete IL-6, which binds to the IL-6R/gp130 complex on hepatocytes to activate JAK; phosphorylated JAK induces STAT3 phosphorylation, and nuclear-translocated p-STAT3 binds to the Ccng1 promoter to promote its transcription; CCNG1 then regulates MDM2 to mediate ubiquitination-dependent TP53 proteolysis, ultimately enhancing hepatocyte apoptosis and driving radiation-induced liver disease (RILD) progression.

Article Snippet: TUNEL staining was performed using a TUNEL BrightGreen Apoptosis Detection Kit (Vazyme, #A112-03) according to the manufacturer's instructions.

Techniques: Irradiation, Phospho-proteomics, Ubiquitin Proteomics

Journal: Redox Report : Communications in Free Radical Research

Article Title: Ligustroflavone alleviates chronic kidney disease by inhibiting ferroptosis through the GSK3β/NRF2 signaling pathway

doi: 10.1080/13510002.2026.2636421

Figure Lengend Snippet: Ligustroflavone attenuates inflammation and death of RTECs caused by UUO. (A) TUNEL staining of renal tissues (magnification 400×; scale bar, 20 μm; green: TUNEL; blue: DAPI). Semi-quantitative analysis of TUNEL-positive cells is shown. (B) Western blot analysis of KIM-1 and NGAL protein expression in renal tissues. Band densities were quantified using ImageJ, with statistical results shown to the right. (C) IHC staining of F4/80 in renal tissues (magnification, 400×; scale bar, 20 μm), Semi-quantitative analysis by ImageJ is shown to the right. (D) Expression of IL1β, IL6, TNFα, and MCP1 genes in renal tissues measured by qRT–PCR. Data are presented as mean ± SD (n = 6). **** P < 0·0001, *** P < 0·001, ** P < 0·01,* P < 0·05 (one-way ANOVA for A, B, C, two-way ANOVA for D).

Article Snippet: Apoptotic cells in kidney tissue sections were detected using the BrightGreen TUNEL kit (A112-01/02/03, Vazyme, China) according to the manufacturer’s instructions.

Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunohistochemistry, Quantitative RT-PCR

Journal: Investigative Ophthalmology & Visual Science

Article Title: YBX1 Modulated Corneal Neovascularization Induced by Alkali Burn via m 5 C-Dependent Regulation of the STAT3/HIF-1α/VEGFA Axis

doi: 10.1167/iovs.67.2.42

Figure Lengend Snippet: YBX1 regulates angiogenesis and apoptosis in HUVECs through the HIF-1α pathway. ( A–H ) Western blot results and quantification of HIF-1α, VEGFA, Bcl-2, Bax, cleaved PARP, and cleaved Caspase-3 expression levels after overexpression of HIF-1α in the context of YBX1 knockdown. ( I ) TUNEL staining of control, H/R and different treatment groups evaluating by fluorescence microscope. Magnification = 40×; Scale bar = 50 µm. ( J ) Double ICC labeling for VEGFA and CD31 in HUVECs with YBX1 knockdown and HIF-1α overexpression. Magnification = 400×; Scale bar = 50 µm. ( K ) Scratch wound healing assay showing changes in cell migration ability among different treatment groups. Magnification = 100×; Scale bar = 500 µm. ( L ) Transwell migration assay assessing invasion capacity after YBX1 knockdown and HIF-1α overexpression. Magnification = 400×; Scale bar = 125 µm. * P < 0.05 versus the control group, # P < 0.05 versus the H/R group, ◆ P < 0.05 versus the H/R + siNC group, & P < 0.05 versus the H/R + siYBX1. Data are presented as mean ± SEM ( n = 6). Means were compared by 1-way ANOVA with Tukey's multiple comparison post hoc tests.

Article Snippet: Cell apoptosis was assessed via TUNEL staining (Vazyme, Cat #A112).

Techniques: Western Blot, Expressing, Over Expression, Knockdown, TUNEL Assay, Staining, Control, Fluorescence, Microscopy, Labeling, Wound Healing Assay, Migration, Transwell Migration Assay, Comparison